Fig. 2

Zebrafish embryos treated with PFOA showed a significant decrease in neutrophil accumulation following wounding. The zebrafish chemotaxis assay showed that PFOA treatment reduced the neutrophil number migrating to the wound site in 48-hpf zebrafish embryos. a–c, Representative images of ISH-tagged neutrophils migrating to the wound region (dotted line), where a wounded embryos treated with vehicle control (DMSO) (n = 36); b wounded embryos treated with PFOA concentration at 0.5 mg/L (n = 35); and c wounded embryos treated with PFOA concentration at 5.0 mg/L (n = 31). d) The averaged quantification of neutrophil migration in each treatment (error bars represent standard deviation across three replicate experiments). Neutrophil migration in both 0.5 and 5.0 mg/L PFOA was significantly decreased when compared to control using ANOVA, with p-values of 0.0025 and < 0.0001, respectively. The DMSO control never exceeded 0.5% v/v and was constant across all treatments. See Additional file 1: File S1 for detailed methodology