ZFIN ID: ZDB-FIG-200901-5
Izarzugaza et al., 2020 - Systems genetics analysis identifies calcium-signaling defects as novel cause of congenital heart disease. Genome Medicine   12:76 Full text @ Genome Med.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 10-13 somites to Long-pec

Fig. 5

Functional validation of candidate genes in zebrafish. a Phenotype of controls and morphants. Single genes and combinations of genes targeted by splicing morpholinos are indicated on the left. Upper panels: gross appearance of 48 hpf zebrafish embryos. Lower panel: examples of cardiac phenotypes of 48 hpf wt, control, and morphant embryos. Hearts were visualized by ISH with a probe against the cardiac marker myl7. b Quantification of phenotypes in wt, controls, morphants, and mRNA rescued morphants. Note the combinatorial effects on cardiac phenotypes when more than one gene is affected. c, d Expression of mef2cb in 10 somite stage zebrafish embryos, analyzed by qRT-PCR (c) and ISH (d). ISH staining intensity was quantified and analyzed using Student’s T test. *p < 0.05, **p < 0.01

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mef2cb AB/TL + MO1-adcy2a standard conditions 10-13 somites whole organism RTPCR
AB/TL + MO1-itpr1b standard conditions 10-13 somites anterior lateral plate mesoderm ISH
10-13 somites whole organism RTPCR
AB/TL + MO1-plcb2 standard conditions 10-13 somites anterior lateral plate mesoderm ISH
10-13 somites whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TL + MO1-adcy2a standard conditions 10-13 somites whole organism mef2cb expression decreased amount, abnormal
Long-pec atrioventricular canal development disrupted, abnormal
Long-pec atrioventricular ring morphology, abnormal
Long-pec determination of heart left/right asymmetry disrupted, abnormal
Long-pec heart inverted, abnormal
Long-pec heart looping disrupted, abnormal
AB/TL + MO1-adcy2a + MO1-itpr1b standard conditions Long-pec atrioventricular canal development disrupted, abnormal
Long-pec atrioventricular ring morphology, abnormal
Long-pec brain edematous, abnormal
Long-pec determination of heart left/right asymmetry disrupted, abnormal
Long-pec heart inverted, abnormal
Long-pec heart looping disrupted, abnormal
AB/TL + MO1-adcy2a + MO1-itpr1b + MO1-plcb2 standard conditions Long-pec atrioventricular canal development disrupted, abnormal
Long-pec atrioventricular ring morphology, abnormal
Long-pec brain edematous, abnormal
Long-pec determination of heart left/right asymmetry disrupted, abnormal
Long-pec heart inverted, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec post-vent region curved, abnormal
AB/TL + MO1-adcy2a + MO1-plcb2 standard conditions Long-pec atrioventricular canal development disrupted, abnormal
Long-pec atrioventricular ring morphology, abnormal
Long-pec determination of heart left/right asymmetry disrupted, abnormal
Long-pec heart inverted, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec post-vent region curved, abnormal
AB/TL + MO1-itpr1b standard conditions 10-13 somites anterior lateral plate mesoderm mef2cb expression decreased amount, abnormal
10-13 somites whole organism mef2cb expression decreased amount, abnormal
Long-pec atrioventricular canal development disrupted, abnormal
Long-pec atrioventricular ring morphology, abnormal
Long-pec brain edematous, abnormal
Long-pec determination of heart left/right asymmetry disrupted, abnormal
Long-pec heart inverted, abnormal
Long-pec heart looping disrupted, abnormal
AB/TL + MO1-itpr1b + MO1-plcb2 standard conditions Long-pec atrioventricular canal development disrupted, abnormal
Long-pec atrioventricular ring morphology, abnormal
Long-pec brain edematous, abnormal
Long-pec determination of heart left/right asymmetry disrupted, abnormal
Long-pec heart inverted, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec post-vent region curved, abnormal
AB/TL + MO1-plcb2 standard conditions 10-13 somites anterior lateral plate mesoderm mef2cb expression decreased amount, abnormal
10-13 somites whole organism mef2cb expression decreased amount, abnormal
Long-pec atrioventricular canal development disrupted, abnormal
Long-pec atrioventricular ring morphology, abnormal
Long-pec determination of heart left/right asymmetry disrupted, abnormal
Long-pec heart inverted, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec post-vent region curved, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Genome Medicine for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genome Med.