FIGURE 2

Recent findings from live imaging secondary angiogenesis/lymphatic sprouting in the zebrafish trunk. (A) Schematic diagram showing events that occur during secondary angiogenesis and anastomosis in the zebrafish trunk. Yellow arrows indicate direction of blood flow. Red vessels are arteries while blue vessels are veins. Light blue cell is a venous endothelial cell (EC) and the green cell is a fated lymphatic EC. (B) Schematic diagram illustrating the difference in Notch activity, cell polarity, and cell arrangements between future arterial intersegmental vessels and venous intersegmental vessels prior to secondary sprout anastomosis. (C) Schematic diagram showing how a Prox1-low EC (light green cell) in the posterior cardinal vein undergo bipotential cell division to give rise to a lymphatic EC (green cell) and a venous EC (light blue cell). aISV, arterial intersegmental vessel; DA, dorsal aorta; LEC, lymphatic endothelial cell; ISV, intersegmental vessel; PCV, posterior cardinal vein; vISV, venous intersegmental vessel.