Fig. 4

Loss of prps1a, not prps1b, generates eye, pigmentation, head, and swim bladder phenotypes. A, Representative bright-field images of wild-type, prps1a mutant, and prps1b mutant embryos at 2 dpf. Reduced eye size is observed in both prps1a mutants, but not in prps1b mutants (n = 16 embryos for each genotype). B, Representative bright-field images of wild-type, prps1a mutant, and prps1b mutant embryos at 5 dpf. Reduced eye and head size, reduced swim bladder inflation, and reduced xanthophore-derived yellow pigmentation are observed in prps1a mutants, but not in prps1b mutants (n = 16 embryos for each genotype). C, Representative bright-field images of wild- type, prps1asd59 mutant, and prps1asd59;prps1bsd61 double mutant embryos at 2 and 5 dpf. No additional phenotypes are observed in the double mutants compared to prps1asd59 mutants alone (n = 12 embryos for each genotype and stage)