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Figure 1

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ZDB-FIG-200803-1

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Calisesi et al., 2020 - Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction

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Figure 1

Experimental setup for multi-view bright-field reconstruction: an LED (530 nm) illuminates the sample, the transmitted light is collected by a detection optical system and a camera. The sample is mounted on a translation and a rotation stage to be scanned and rotated around 360°. A stack of images is acquired in each angular position while scanning the sample (a). Scheme of the Optical Transfer Function of the microscope (b, upper panel). Acquiring multiple views is equivalent to rotating the OTF and sampling different spatial frequencies (b, lower panel). Reconstruction of the transverse section of a sample (Arabidopsis thaliana) using a single view (0°), 10 views (covering 60° in total) and 60 views (covering 360°), each with a spacing of 6°. The illumination and detection numerical aperture is NA = 0.13 (c). Scale bar: 50 µm.

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