Figure 8

Loss of her9 has minimal effects other retinal cell types. Whole eye images of WT and heterozygous embryos (A) or her9 mutant embryos (B) on the Ath5:GFP transgenic background at 5 dpf. Immunohistochemistry for ganglion and amacrine cells (HuC/D antibody) in cryosections from WT and heterozygous retinas (C–D) or her9 mutant retinas (E–F). Ganglion cell: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p < .0001). Horizontal cells: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p = .455). Amacrine cells: WT/Het = 10 embryos; Mut = 10 embryos; t-test (p = 0.5). (G–H′) Immunohistochemistry with the Prox1 antibody labels horizontal cells in cryosections of WT and heterozygous (G–H′) or her9 mutant (I–J′) retinas. Cell counts reveal a decrease in the number of ganglion cells in the mutant retinas (K) but no significant difference in the number of horizontal or amacrine cells (L-M). Am, Amacrine cells; GC, Ganglion cells; Hz, Horizontal cells; ONL, outer nuclear layer; INL, inner nuclear layer; ON, optic nerve; L, Lens. Scale bar = 50 µm and 100 µm.