FIGURE

Fig. 1.

ID

ZDB-FIG-200703-26

Publication

Sam et al., 2020 - Specificity, redundancy and dosage thresholds among gata4/5/6 genes during zebrafish cardiogenesis

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Fig. 1.

Mutation of gata4 is tolerated while mutationsin gata5 or gata6 cause embryonic lethal cardiac defects. (A–E) Representative images of 2 dpf gata4, gata5, or gata6 homozygous mutants compared to wild type following TALEN (gata4 and gata6) or CRISPR (gata5) mediated deletion of the GATA zinc finger DNA-binding domain. (B,C) Gata4 homozygous mutants are phenotypically normal. (D,E) Gata5 and gata6 homozygous mutants develop severe pericardial edemas (indicated by white arrows in the far right panels) and fail to survive beyond 5–6 days. These phenotypes are 100% penetrant. Representative scale bar in A: 0.5 mm. (F–H) Schematic showing the structure, partial nucleotide sequence, and partial amino acid sequence for each of the gata4/5/6 mutant alleles. Zinc finger domains are shown in yellow and regions targeted for deletion are marked by dashed lines.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gata4^wcm5/wcm5 gata4^wcm6/wcm6 gata5^wcm8/wcm8 gata6^wcm7/wcm7
Observed In:	pericardium whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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