Fig. 2

Human cancer cell lines (Hs578T, MDA-MB-468, HCT116, SW620 or HT29) were injected into the PVS of 2 dpf Tg(fli1:eGFP) zebrafish larvae. Zebrafish xenografts were treated in vivo with bevacizumab and compared with untreated controls. At 4 dpi, zebrafish xenografts were imaged by confocal microscopy (a–e’). The percentage of mitotic figures (f), apoptosis (g, ****P < 0.0001) and tumor size (h, ****P < 0.0001) were quantified. The outcomes are expressed as AVG ± SEM. The number of xenografts analyzed are indicated in the representative images and each dot represents one zebrafish xenograft. Results are from 3 (Hs578T and MDA-MB-468) and 2 (HCT116, SW620 and HT29) independent experiments, which are highlighted in different colors corresponding to each individual experiment. Statistical analysis was performed using an unpaired t-test. Statistical results: (ns) > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Scale bars represent 50 μm. All images are anterior to the left, posterior to right, dorsal up and ventral down.