FIGURE

Fig. 6

ID

ZDB-FIG-200619-27

Publication

Chestnut et al., 2020 - Single-cell transcriptomic analysis identifies the conversion of zebrafish Etv2-deficient vascular progenitors into skeletal muscle

Other Figures

(all 9)

All Figure Page

Back to All Figure Page

Fig. 6

Wnt and FGF signaling is required for differentiation of <italic>etv2</italic><sup><italic>ci32Gt</italic></sup><italic>; UAS:GFP</italic> cells into skeletal muscle.

a–c Heat-shock inducible expression of Wnt inhibitor Dkk1 greatly reduces GFP-positive muscle cells in hsp70:dkk1; etv2^ci32Gt+/−; UAS:GFP embryos. Heat-shock (HS) was performed at the 8-somite stage, and GFP fluorescence imaged at 24–26 hpf. Sixteen embryos were analyzed in each group in two replicate experiments. d–fetv2^{ci32Gt−/−}; UAS:GFP embryos treated with FGF inhibitor SU5402 starting at the 50% epiboly stage show a greatly reduced number of GFP-positive cells at the 22-somite stage. Nine control DMSO-treated and 15 SU5402-treated embryos were analyzed in two replicate experiments. g–i Heat-shock inducible expression of dnFGFR1 results in a significant reduction of mCherry-positive muscle cells in etv2^ci32Gt+/−; UAS:NTR-mCherry; hsp70:dnFGFR1 embryos. Twenty each control and HS hsp70:dnFGFR1 embryos were analyzed in two replicate experiments. Median ± SD values are shown in all graphs, two-tailed Student’s t test was used for statistical analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	etsrp^{ci32Gt/ci32Gt}; nkuasgfp1aTg etsrp^ci32Gt/+; nkuasgfp1aTg; w32Tg etsrp^ci32Gt/+; c264Tg; pd1Tg
Conditions:	chemical treatment by environment: SU5402 heat shock
Observed In:	skeletal muscle cell differentiation
Stage Range:	20-25 somites to Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.