FIGURE

Fig. 6

ID

ZDB-FIG-200619-27

Publication

Chestnut et al., 2020 - Single-cell transcriptomic analysis identifies the conversion of zebrafish Etv2-deficient vascular progenitors into skeletal muscle

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Fig. 6

Wnt and FGF signaling is required for differentiation of <italic>etv2</italic><sup><italic>ci32Gt</italic></sup><italic>; UAS:GFP</italic> cells into skeletal muscle.

a–c Heat-shock inducible expression of Wnt inhibitor Dkk1 greatly reduces GFP-positive muscle cells in hsp70:dkk1; etv2^ci32Gt+/−; UAS:GFP embryos. Heat-shock (HS) was performed at the 8-somite stage, and GFP fluorescence imaged at 24–26 hpf. Sixteen embryos were analyzed in each group in two replicate experiments. d–fetv2^{ci32Gt−/−}; UAS:GFP embryos treated with FGF inhibitor SU5402 starting at the 50% epiboly stage show a greatly reduced number of GFP-positive cells at the 22-somite stage. Nine control DMSO-treated and 15 SU5402-treated embryos were analyzed in two replicate experiments. g–i Heat-shock inducible expression of dnFGFR1 results in a significant reduction of mCherry-positive muscle cells in etv2^ci32Gt+/−; UAS:NTR-mCherry; hsp70:dnFGFR1 embryos. Twenty each control and HS hsp70:dnFGFR1 embryos were analyzed in two replicate experiments. Median ± SD values are shown in all graphs, two-tailed Student’s t test was used for statistical analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	etsrp^{ci32Gt/ci32Gt}; nkuasgfp1aTg etsrp^ci32Gt/+; nkuasgfp1aTg; w32Tg etsrp^ci32Gt/+; c264Tg; pd1Tg
Conditions:	chemical treatment by environment: SU5402 heat shock
Observed In:	skeletal muscle cell differentiation
Stage Range:	20-25 somites to Prim-5

Phenotype Detail

Acknowledgments

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