Fig. 4

a,b Quantification (branch length and number of intersection points) of capillary-like structure formation in A549 (a) and H1299 (b) cells plated on matrigel and treated with 20 μM CPTH6 for 18 h. The results represent the average ± SD of two independent experiments. c Representative images of immunofluorescence conducted in A549 and H1299 cells control or treated with 50 μM CPTH6 for 24 h using rhodamine-conjugated phalloidin to visualize F-actin fibers or for vinculin expression and localization. Blue fluorescence represents DAPI stained nuclei. d Representative images and relative quantification of migration of A549 cells control or treated with 50 μM CPTH6 for 48 h and subjected to time-lapse videorecording. The images were recorded every 15 min, and were taken at the starting point (0 h), after 24 and 36 h. The red outlines show the gap area. The migration rates of two different conditions (control and CPTH6) were determined as the percentage of wound closure or the percentage of area reduction. Magnification 10X. Scale bar, 100 μm. a, b, d p values were calculated between control and CPTH6-treated cells. *p < 0.05; **p < 0.01