FIGURE

Figure 2

ID

ZDB-FIG-200608-2

Publication

Albadri et al., 2020 - Expression of a Barhl1a reporter in subsets of retinal ganglion cells and commissural neurons of the developing zebrafish brain

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Tg(barhl1a:GFP) labels new-born RGCs. (A–D) Spatial-temporal distribution of Tg(barhl1a:GFP) expression in three different retinal developmental stages at 30 (A), 32 (B), 34 (C) and 36 (D) hpf. The GFP signal (green) becomes first evident at ~30 hpf in the ventral retina (white arrow in A) and subsequently follows the spatio-temporal wave of RGC differentiation. All images represent 1 µm Z-stacks in lateral view. (E–G’) Single 1 µm optical slice (Z-stack) of a 33 hpf Tg(barhl1a:GFP) embryo immunolabeled with anti-Cxcr4b (magenta) and anti-GFP (green) antibodies. (E) GFP is detected in cells located in the basal half of the neuroepithelium around the lens at 33 hpf. These are also labelled by Cxcr4b (magenta in F, G and zoomed insert G’), which mark post-mitotic RGCs and their precursors at the cell membrane. Asterisks in G’ highlight Cxcr4b-positive (GFP-negative) RGCs. All images represent lateral view, anterior is left, dorsal is top. Scale bars: 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.