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ZDB-FIG-200604-11

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Green et al., 2020 - Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs

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Fig. 3

Wnt suppression during late epiboly/early segmentation leads to progressive loss of mes/r1 and expansion of forebrain. In situ hybridization to fgf8a (A–F), pax2a (G–L), en2a (M–R), and wnt1 (S–X) on hs:dkk1b/+ or +/+ sibling embryos. Dorsal views, anterior up (A,B,G,H,M,N,S,T). Lateral views, dorsal right, anterior up (C–F, I-L, O-R, U-X). Note normal fgf8a and pax2a at 10.5 hpf, despite significant reduction of wnt1 and en2a. By 14 hpf, MHB expression of fgf8 and pax2a is lost (indicated by black asterisks, C,I,E,K absent in D,J,F,L) with posterior expansion of telencephalic fgf8a (red asterisks, C–F) and optic stalk pax2a (arrows, I-L). At 16 hpf, posterior expansion of forebrain has increased (E,F,K,L). Midbrain and MHB wnt1 and en2a expression is absent, while dorsal hindbrain and spinal cord wnt1 persists (large arrows, V,X). .

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Acknowledgments

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Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.