FIGURE

Figure 3

ID

ZDB-FIG-200517-21

Publication

Zhou et al., 2020 - A T3SS Regulator Mutant of Vibrio alginolyticus Affects Antibiotic Susceptibilities and Provides Significant Protection to Danio rerio as a Live Attenuated Vaccine

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Figure 3

Construction and confirmation of the knockout mutant strain HY9901ΔtyeA. (A) M: DL2000 marker; Lane 1. The 549 bp upstream fragment amplified from genomic DNAs of the strain HY9901 using primer pairs of tyeA-for/tyeA-int-rev; Lane 2. The 543 bp downstream fragment amplified from genomic DNAs of the strain HY9901 using primer pairs of tyeA-int-for / tyeA-rev. (B) M: DL2000 marker; Lane 1. The 1,092 bp fragment amplified from genomic DNAs of HY9901ΔtyeA using primer pairs of tyeA-for/tyeA-rev. (C) M: DL2000 marker; Lane 1–7. The 1,104 bp fragment amplified from genomic DNAs of the HY9901ΔtyeA using primer pairs of tyeA-for/tyeA-rev; Lane 8. The 1,329 bp fragment amplified from genomic DNAs of the wild-type strain HY9901 using primer pairs of tyeA-for/tyeA-rev. (D) M: DL2000 marker; Lane 1–10. The 1,252 bp fragment amplified from genomic DNAs of HY9901ΔtyeA; Lane11. The 1,489 bp fragment amplified from genomic DNAs of the strain HY9901 using primer pairs of tyeA-up / tyeA-down.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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