Fig. s6
Loss of prdm16 causes a subtle decrease in expression of neural crest markers dlx2a or crestin in the pharyngeal arches. (A-B) Wildtype or prdm16−/− mutant embryos were collected and in situ hybridization for dlx2a (A) or crestin (B) was performed at 24 hpf. Shown are lateral views of dlx2a expression in the pharyngeal arches (1–4) (A) and lateral views of crestin across the whole body to show neural crest migration is unchanged in prdm16 mutant zebrafish. Higher magnification, dorsal views of crestin expression is also shown in (B). Scale bars, 100 μm. Abbreviations: eye (e), 1–4 (pharyngeal arches 1–4), posterior pharyngeal arches (ppa). |
Reprinted from Developmental Biology, 461(2), Shull, L.C., Sen, R., Menzel, J., Goyama, S., Kurokawa, M., Artinger, K.B., The conserved and divergent roles of Prdm3 and Prdm16 in zebrafish and mouse craniofacial development, 132-144, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.