Fig. 6

Junction elongation and JBL formation are functionally linked. a Still images from a movie of an VE-cad-Venus expressing embryo Tg(BAC(cdh5:cdh5-ts)) during anastomosis in the DLAV (around 32?hpf), in the presence of DMSO (1%), Latrunculin B (150?ng?ml?1) or NSC23766 (900?µM). b Scatter plot quantitation of the duration of the JBL. DMSO, n?=?50 (6 movies); Latrunculin B, n?=?13 (5 movies); NSC23766, n?=?49 (10 movies); black lines show median values. Non-parametric Kruskal?Wallis statistical test was used. c Confocal images of a Tg(BAC(cdh5:cdh5-ts)) embryo during junctional elongation after DLAV anastomosis. Top panels t?=?0 and bottom panels after 1?h incubation. d Quantification of the junctional elongation velocity in the presence of different chemicals using Tg(BAC(cdh5:cdh5-ts)) embryos. DMSO (1%), n?=?29 junctions (11 embryos); Latrunculin B (150?ng?ml?1), n?=?21 (6 embryos); NSC23766 (300?µM), n?=?41 (11 embryos). Dotted line indicated no movement observed, black lines are medians. Non-parametric Kruskal?Wallis statistical test was used. e Confocal images of anastomosing DLAV of EGFP-ZO1 embryos (Tg(fli:Gal4ffubs3;UAS:EGFP-hZO1ubs5)) treated with DMSO or NSC23766. Scale bar 10?µm