Fig. 3

Histological identification of lymphatic ducts labeled with cinnabar. A, Lateral lymphatic duct system of the pflt4‐EGFP adult medaka (left; cranial and top; dorsal). Subcutaneous lymphatic duct was labeled with cinnabar dye in the left trunk. B, Magnified cross‐section (hematoxylin‐eosin) of the duct labeled with cinnabar particles (arrow) at the level indicated by the white bar in Figure 3A (right; lateral and top; dorsal, LL, lateral line; Ll, lateral lymphatic duct). C, Lymphatic ducts in the trunk and tail of the pflt4‐EGFP adult medaka (left; cranial and top; dorsal). Deep lymphatic ducts (Carl‐Caul, cardinal‐caudal lymphatic; Sl, spinal lymphatic) and lymphatic vascular system in appendages (Afl, Anal fin lymphatic; Dfl, dorsal fin lymphatic; Pefl, pelvic fin lymphatic) were labeled with cinnabar dye. D, Cinnabar particles (blackish pigments) adhered to the EGFP‐immunopositive endothelia lining of the spinal lymphatic duct (blue) in the cross‐section of pflt4‐EGFP medaka. E, Lymphatic ducts in the trunk and tail of the adult zebrafish (left; cranial and top; dorsal). Deep lymphatic ducts (Carl‐Caul, cardinal‐caudal lymphatic; Sl, spinal lymphatic) and lymphatic vascular systems in appendages (Afl, Anal fin lymphatic; Cfl, caudal fin lymphatic), Dfl, dorsal fin lymphatic; Pefl, pelvic fin lymphatic) were labeled with cinnabar dye. F, Prox1 immunostaining for transversely sectioned lymphatic vessels in the pelvic fin of cinnabar‐injected zebrafish. Cinnabar particles (arrows) accumulated around the Prox1‐immunopositive vessels (blue). Histochemical controls in respective sections adjacent to D,F. Bars, 50 μm