Figure 3

Microcin E492 intratumoral injection reduces the tumor size in zebrafish larvae SW620 xenograft model. (A) Schematic representation of the experimental setup for the development of zebrafish xenografts using SW620 human colorectal cancer cells to test the MccE492 antitumoral effect. (B) Assessment of the tumor cell mass size through live-cell imaging in zebrafish xenografts upon injection of MccE492, a mock purification, or PBS. Representative images of each condition are shown in the left side. The photographed area is delineated in the 5-dpf larva scheme shown. The insets show the saturated fluorescence area used in the estimation of tumors size (only the zones inside the white dashed lines were considered, as the excluded red zones correspond to reflections of the fluorescence signal in the swim bladder). The relative tumor size, corresponding to the percent change in tumor size comparing the same individual at 24 and 72 hpt, was determined and plotted for a total cohort of 50 individuals per condition. Error bars correspond to the standard deviation. ****P < 0.0001 (two-way ANOVA with a Dunnet's post-test).