Fig. 5

Rho-GTPases Drive Yap Mechanotransduction and HSPC Production In Vivo (A) WISH for runx1/cmyb at 36 hpf in WT embryos after treatment (12–36 hpf) with Rho-GTPase activators CN02, CN03, and CN04. Scale bar, 100 μm. (B) Distribution plot of embryos in (A) (n ≥ 50 per condition; chi-squared test, ∗p < 0.05, ∗∗∗p < 0.001). (C) qPCR after treatment with CN02 or CN04 (unpaired Student’s t test versus control, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Error bars indicate SEM). (D) Quantification of CD41:GFP+ cells in images of the CHT at 72 hpf (n = 30 control, 23 CN02, and 22 CN04; unpaired Student’s t test; ∗∗∗p < 0.001. Error bars indicate SD). (E) WISH for runx1/cmyb at 36 hpf in sih morphants after CN02 treatment (12–36 hpf). Scale bar, 100 μm. (F) Distribution plot of embryos in (F) (n ≥ 50 per condition; chi-squared test, ∗∗∗p < 0.001). (G) qPCR in WT embryos and sih morphants at 36 hpf after CN02 treatment (unpaired Student’s t test, ∗∗p < 0.01, ∗∗∗p < 0.001. Error bars indicate SEM).