Figure 2

Conservation of tilapia mstn2. (A) Chromosomal synteny of Nile tilapia mstn1 and mstn2. Genes adjacent to mstn in the Nile tilapia, zebrafish and human genomes were manually identified using both USCS and ensembl genome browsers (https://genome.ucsc.edu/ and https://www.ensembl.org/index.html, respectively). The genes are named according to their annotation in the human genome. While Nab1 and MFSD6a genes were found to be syntenic in both piscine mstn genomic regions, most of the human MSTN neighboring genes were syntenic only to mstn1 or mstn2. (B) Pairwise alignment of tilapia Mstn1 and Mstn2 amino acid sequences illustrates the high similarity of the tilapia Mstn proteins. Red asterisk indicates conserved cysteine residues, which are important for Mstn peptide activity. The conserved proteolytic RXRR motif is indicated by a red rectangle.