Figure 3

Primary Screen results for representative small molecule inhibitors. (A–K) Expression of the DA and NA neuronal marker th detected by whole mount in situ hybridization in embryos fixed after drug or control treatment at 96 hpf. Treatments are indicated above each panel. During the primary screen th expression was analyzed in DA clusters in telencephalon, pretectum, and diencephalic groups 1–6. Dorsal views of heads of larvae, images generated from Z-Projections of image stacks. Neuronal groups with reduced WISH stain are indicated as follows: Black arrowheads for telencephalic DA clusters (B–G, J, K), black arrows for pretectal DC clusters (B, C, E–G), black asterisks for DC DA clusters (B, C, E). White asterisks indicate increased stain intensities in DC DA clusters (H–K). Scale bar in (A) is 100 µm for all panels. AC, amacrine cells; Tc, telencephalon; PrT, pretectum; DC, diencephalic groups; Lc, locus coeruleus; MO/AP, medulla oblongata/area postrema.