Fig. 5

Proliferation of cells in the optic tectum of  scn1labmut/mut larvae. A, At 6 days postfertilization (dpf),  scn1labmut/mut larvae showed an increased number of proliferating cells in the optic tectum, as measured by BrdU?positive cells in 30??m?thick optical sections. Incubation of larvae in 50 ?mol/L of fenfluramine (FEN) from 3 dpf onward resulted in an increased number of BrdU?positive cells in wild?type (WT) larvae at 6 dpf, while reducing the number to untreated WT levels in the  scn1labmut/mut larvae. Statistical significance was calculated using multiple unpaired, two?tailed  t tests. * P < .05, ** P < .01, **** P < .0001. B, Cartoon representation of the head of a 6?dpf larva, indicating the position of the optic tectum where proliferation was measured. C, Representative z?slices of masked optic tecta with BrdU staining of WT and  scn1labmut/mut larvae at 6 dpf, with or without prior incubation in 50 ?mol/L of FEN from 3 dpf onward. Squares indicate the location of the zoomed?in, split?channel images to the right. Scale bars = 50 ?m