FIGURE

Fig. S2

ID

ZDB-FIG-200326-29

Publication

Giusti et al., 2019 - Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound

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Fig. S2

duox mutant fish are affected by thyroid dyshormonogenesis. (A) Target site for duox gRNA located in the exon 23 (CGG PAM sequence is underlined). The duox mutated sequences of the three mutant fish lines presenting deletions of 1, 3 or 10 nucleotides are shown. Frame-shift mutations in duox Δ1nt and Δ10nt mutant lines generate premature stop codons after residues 1078 and 1075, respectively. (B) Proportion of larvae (120 hpf) presenting a goitrous thyroid phenotype as detected in the progeny of mating experiments of heterozygous duox Δ1nt, Δ3nt and Δ10nt mutant fish, respectively. (C) WIF staining for thyroid EGFP reporter and colloidal T4 of 120 hpf duox Tg(tg:nlsEGFP) mutants showed important reduction of detectable T4 signal and thyroid enlargement in all homozygous (−/−) duox mutant larvae (eight specimens per genotype analyzed). Representative epifluorescence images are shown (ventral views, anterior to the left, scale bars: 75 μm).

Expression Data

Gene:	EGFP
Antibody:	Ab-T4
Fish:	duox^{zf3298/zf3298}; ulb4Tg duox^{zf3299/zf3299}; ulb4Tg duox^{zf3300/zf3300}; ulb4Tg duox^{sa13017/sa13017}; mitfa^w2/w2; mpv17^a9/a9; ulb4Tg
Anatomical Term:	thyroid follicle
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	duox^{zf3298/zf3298}; ulb4Tg duox^{zf3299/zf3299}; ulb4Tg duox^{zf3300/zf3300}; ulb4Tg duox^{sa13017/sa13017}; mitfa^w2/w2; mpv17^a9/a9; ulb4Tg
Observed In:	thyroid follicle
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 500, Giusti, N., Gillotay, P., Trubiroha, A., Opitz, R., Dumont, J.E., Costagliola, S., De Deken, X., Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound, 110635, Copyright (2019) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.