ZFIN ID: ZDB-FIG-200323-44
Depienne et al., 2019 - Association of Rare Genetic Variants in Opioid Receptors with Tourette Syndrome. Tremor and other hyperkinetic movements (New York, N.Y.)   9 Full text @ Tremor Other Hyperkinet Mov (N Y)
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2

Knockdown of the Zebrafish OPRK1 Orthologue Results in Hyperactivity during Development. (A) Activity heatmap illustrating sponta- neous movement of 28 hpf embryos inside the chorion during a 10 seconds period. Higher levels of activity are evident in embryos injected with oprk1 splice-blocking AMO (spOPRK1) or ATG-targeting AMO (OPRK1 KD) when compared to mismatch AMO-injected embryos (Mismatch control). Expression of human OPRK1 transcript (hOPRK1) rescues the hyperactivity phenotype. (B) RT-PCR product of amplification of a region spanning exons 2 and 3 of the zebrafish oprk1 transcript. Injection of the splice-blocking AMO (spOPRK1) results in a defective splicing in this region. (C) Quantification of the total activity of 28 hpf zebrafish embryos. (D) Quantification of the frequency of movement bursts in the 28 hpf embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-oprk1 standard conditions Prim-5 locomotory behavior process quality, abnormal
Prim-5 whole organism increased behavioural activity, abnormal
WT + MO2-oprk1 standard conditions Prim-5 locomotory behavior process quality, abnormal
Prim-5 whole organism increased behavioural activity, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Tremor and other hyperkinetic movements (New York, N.Y.) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Tremor Other Hyperkinet Mov (N Y)