FIGURE

Fig. 4

ID

ZDB-FIG-200320-4

Publication

Chou et al., 2018 - Ribose-5-Phosphate Isomerase A Overexpression Promotes Liver Cancer Development in Transgenic Zebrafish via Activation of ERK and β-catenin Pathways

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

pERK expression is elevated in RPIA-transgenic fish. (A) Representative images of pERK IHC results for the control (mC), and two RPIA-transgenic fish (Tg1 and Tg2) aged 7?11 months. The control fish displayed only a weak pERK signal. The 7- to 11-month-old Tg1 and Tg2 fish exhibited significant stronger staining. All the magnifications were ×400. Scale bar: 50 ?m. (B) Statistical analysis of the IHC results for pERK expression of two independent Tg (fabp10a: RPIA)?Tg1 and Tg2 compared with control (mC) fish. Statistical analysis was performed by unpaired two-tailed t-tests. Asterisks (*) represent the level of significance. *P < 0.05 was considered statistically significant. **P < 0.01; ***P < 0.001. (C) Immunofluorescence staining of pERK in two independent RPIA-transgenic zebrafish (Tg1 and Tg2) compared with control (mC). The images showed that pERK (green) and DAPI (blue) immunofluorescent signals co-localized within nucleus in RPIA-transgenic zebrafish liver. All of the magnifications were ×400. Scale bar: 40 ?m.

Expression Data

Antibody:	Ab9-mapk
Fish:	nn1000Tg nn1009aTg nn1009bTg
Anatomical Terms:	liver liver nucleus
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nn1009aTg nn1009bTg
Observed In:	liver nucleus
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Carcinogenesis