Fig. 3
- ID
- ZDB-FIG-200319-29
- Publication
- Brantley et al., 2019 - In vivo assessment of gonad status, secondary sex characteristics and spawning in transparent Casper zebrafish
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Effects of diethylstilbestrol (DES) on breeding tubercles of adult male Casper zebrafish. Panels A – C show pectoral fins of same control treated male. Panel A: control male at 17 WPF. Panel B: control male at 20 WPF. Panel C: control male at 22 WPF. Panels A – C show no reduction in breeding tubercles over time. Panels D – F show pectoral fins of same 0.01 μg/L DES treated male. Panel D: DES male at 17 WPF, the day of treatment, before 0.01 μg/L DES was applied. Panel E: DES male at 20 WPF after DES treatment for 21 days. Panel F: DES male at 22 WPF, 14 days after DES withdrawal. Panels D – F show a modest reduction in the height and pointedness of the breeding tubercles after treatment. Panels G – I show pectoral fins of same male treated with 0.1 μg/L DES. Panel G: DES male at 17 WPF, the day of treatment, before 0.1 μg/L DES was applied. Panel H: DES male at 20 WPF after 21 days of DES treatment. Panel I: DES male at 23 WPF, 21 days after DES withdrawal. Images show a clear reduction in breeding tubercles after treatment and reformation with DES withdrawal. Images were taken at 32× magnification; all scale bars represent 400 μm, except inset which are 64× and scale bar represents 200 μm. |
Reprinted from Mechanisms of Development, 160, Brantley, N., Lessman, C.A., In vivo assessment of gonad status, secondary sex characteristics and spawning in transparent Casper zebrafish, 103582, Copyright (2019) with permission from Elsevier. Full text @ Mech. Dev.