FIGURE

Figure 6

ID

ZDB-FIG-200314-23

Publication

Grzegorski et al., 2020 - Disruption of the kringle 1 domain of prothrombin leads to late onset mortality in zebrafish

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Figure 6

Loss of thrombin activity results in defects in secondary hemostasis. (A) Larvae were immobilized in agarose, subjected to laser-mediated endothelial injury (green arrow) of the venous (PCV, blue) or arterial (dorsal aorta, red) circulation, and followed for 2 minutes by a blinded observer. (B) Genetic ablation of f2 resulted in the inability to form induced PCV thrombi at 3 dpf and was not influenced by inhibiting fibrinolysis (ɛ-aminocaproic acid treatment, blue). (C) Overexpression of human F2 cDNA (blue) rescued the ability to form thrombi in the PCV at 3 dpf. (D) Homozygous mutant larvae demonstrated a significant impairment in arterial thrombus formation at 5 and 6 dpf without any changes in the time to initial thrombocyte attachment (E). (F) The number of thrombocytes attached to the site of injury in 2 minutes was significantly increased at 6 dpf in f2 homozygous mutants. Statistical significance assessed by Mann-Whitney U testing.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f2^{mi4008/mi4008} (AB/TL) f2^{mi4008/mi4008}; la2Tg (AB/TL)
Conditions:	light damage: dorsal aorta chemical treatment by environment: 6-aminohexanoic acid, light damage: posterior cardinal vein light damage: posterior cardinal vein
Observed In:	blood coagulation dorsal aorta blood coagulation, fibrin clot formation posterior cardinal vein blood coagulation, fibrin clot formation
Stage Range:	Protruding-mouth to Day 6

Phenotype Detail

Acknowledgments

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