ZFIN ID: ZDB-FIG-200314-23
Grzegorski et al., 2020 - Disruption of the kringle 1 domain of prothrombin leads to late onset mortality in zebrafish. Scientific Reports   10:4049 Full text @ Sci. Rep.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Protruding-mouth to Day 6

Figure 6

Loss of thrombin activity results in defects in secondary hemostasis. (A) Larvae were immobilized in agarose, subjected to laser-mediated endothelial injury (green arrow) of the venous (PCV, blue) or arterial (dorsal aorta, red) circulation, and followed for 2 minutes by a blinded observer. (B) Genetic ablation of f2 resulted in the inability to form induced PCV thrombi at 3 dpf and was not influenced by inhibiting fibrinolysis (ɛ-aminocaproic acid treatment, blue). (C) Overexpression of human F2 cDNA (blue) rescued the ability to form thrombi in the PCV at 3 dpf. (D) Homozygous mutant larvae demonstrated a significant impairment in arterial thrombus formation at 5 and 6 dpf without any changes in the time to initial thrombocyte attachment (E). (F) The number of thrombocytes attached to the site of injury in 2 minutes was significantly increased at 6 dpf in f2 homozygous mutants. Statistical significance assessed by Mann-Whitney U testing.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
f2mi4008/mi4008(AB/TL) light damage: posterior cardinal vein, chemical treatment by environment: 6-aminohexanoic acid Protruding-mouth blood coagulation process quality, abnormal
Protruding-mouth posterior cardinal vein blood coagulation, fibrin clot formation delayed, abnormal
f2mi4008/mi4008(AB/TL) light damage: posterior cardinal vein Protruding-mouth blood coagulation process quality, abnormal
Protruding-mouth posterior cardinal vein blood coagulation, fibrin clot formation delayed, abnormal
f2mi4008/mi4008; la2Tg(AB/TL) light damage: dorsal aorta Day 5 blood coagulation process quality, abnormal
Day 5 dorsal aorta blood coagulation, fibrin clot formation delayed, abnormal
Day 6 blood coagulation process quality, abnormal
Day 6 dorsal aorta blood coagulation, fibrin clot formation delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.