Figure 5

Oxytocin signaling is differently involved in the perception of biological motion and conspecific form. (a) Generation of germline transmitting oxtr mutant. TALEN sites targeting the 1^st exon of the oxtr gene. Single bp mutation generates a truncated receptor. (b) Different concentrations of synthetic isotocin, also known as oxytocin in fish literature, increased cAMP reporter activity of the WT-transfected OXTR, but not the mutant. (c) WT (oxtr^(+/+)) and oxtr mutant (oxtr^(−/−)) fish are allowed to choose between a static fish image (FISH) vs. a static dot (DOT). (d) Comparison of exploratory score between oxtr^(+/+) (grey bars, n = 17) and oxtr^(−/−) (cyan bars, n = 16). (e) Comparison of preference score towards social cues between oxtr^(+/+) (grey bars, n = 17) and oxtr^(−/−) (cyan bars, n = 16). (f) Comparison of mean speed between oxtr^(+/+) (grey bars, n = 17) and oxtr^(−/−) (cyan bars, n = 16). (g) Fish oxtr^(+/+) and oxtr^(−/−) are allowed to choose between a dot moving with biological motion (DOT BM) vs. a non-biological motion (DOT NBM). (h) Comparison of exploratory score between oxtr^(+/+) (grey bars, n = 24) and oxtr^(−/−) (cyan bars, n = 24). (i) Comparison of preference score towards social cues between oxtr^(+/+) (grey bars, n = 24) and oxtr^(−/−) (cyan bars, n = 24). (j) Comparison of mean speed between oxtr^(+/+) (grey bars, n = 24) and oxtr^(−/−) (cyan bars, n = 24). (k) Fish oxtr^(+/+) and oxtr^(−/−) are allowed to choose between a fish moving with biological motion (FISH BM) vs. a fish image moving with non-biological motion (FISH NBM). (l) Comparison of exploratory score between oxtr^(+/+) (grey bars, n = 17) and oxtr^(−/−) (cyan bars, n = 18). (m) Comparison of preference score towards social cues between oxtr^(+/+) (grey bars, n = 17) and oxtr^(−/−) (cyan bars, n = 18). (n) Comparison of mean speed between oxtr^(+/+) (grey bars, n = 17) and oxtr^(−/−) (cyan bars, n = 18). (o) Fish oxtr^(+/+) and oxtr^(−/−) are allowed to choose between a fish moving with biological motion (FISH BM) vs. a dot with non-biological motion (DOT NBM). (p) Comparison of exploratory score between oxtr^(+/+) (grey bars, n = 10) and oxtr^(−/−) (cyan bars, n = 10). (q) Comparison of preference score towards social cues between oxtr^(+/+) (grey bars, n = 10) and oxtr^(−/−) (cyan bars, n = 10). (r) Comparison of mean speed between oxtr^(+/+) (grey bars, n = 10) and oxtr^(−/−) (cyan bars, n = 10). Error bars indicate SEM. *P < 0.05.