Figure 3

Gross analysis with Z-stack images of anti-GFAP labeling in the olfactory bulb following repetitive peripheral damage. (A) Illustration of the treatment paradigm shows that fish receiving repeated wax plug insertions were analyzed at various survival times during wax treatment and after a recovery period. Images of olfactory organs show typical rosette morphology in control fish (B), significant damage at 1 day (C) and 7 days (D) of wax plug insertions, and return to control morphology with 1 week of recovery (E) after removal of the plug. (F) Untreated control fish had typical anti-GFAP labeling in all layers of both olfactory bulbs. Tel = Telencephalon, n = 4. (G) After 1 day of repetitive peripheral damage to the olfactory organ, the affected bulb (asterisk) appeared to have slightly more anti-GFAP labeling than the internal control bulb, n = 3. (H) By 7 days, there was no noticeable difference in labeling between bulbs, and anti-GFAP labeling in the affected bulb (asterisk) appeared at control levels, n = 4. Scale bar = 100 μm for all.(I) A comparison of anti-GFAP labeling between bulbs showed a significant difference at 1 day of repetitive peripheral damage when compared to controls but no difference at 7 days of damage. *p < 0.05.