FIGURE

Figure 4

ID

ZDB-FIG-200302-47

Publication

Petree et al., 2020 - MultiFRAGing: Rapid and Simultaneous Genotyping of Multiple Alleles in a Single Reaction

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Figure 4

Validation of indels by Sanger Sequencing. Each indel that was identified by fragment analysis was sequenced using the Sanger method to establish the correlation between fragment analysis and Sanger sequencing Data. All indels from fragment analysis showed similar indel size in Sanger sequencing. (A) Alignment and chromatogram confirming a 2 bp deletion in dfnb31a. (B) Alignment and chromatogram confirming a 13 bp deletion in dfnb31b. (C) Alignment and chromatogram confirming a 1 bp deletion in grhl2a. (D) Alignment and chromatogram confirming a 10 bp deletion in grhl2a. (E) Alignment and chromatogram confirming a 4 bp insertion in grhl2b.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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