Figure 4

Comparison of localised and systemic Mycobacterium marinum (Mm) infection. (a) Representative images are shown for zebrafish larvae four days after either localised hindbrain ventricle (HBV) infection (n = 14) or systemic intravenous (IV) infection (n = 15) with 100 cfu Mm. Scale bars, 500 µm. Existing outcome measures, integrated fluorescence (IF), a surrogate for total bacterial burden (b) and the number of fluorescent bacterial foci (c) and dissemination parameters, fluor₅₀ (d), grid analysis (e), polygon area (f) and IFD_max (g) are presented. Data points represent individual zebrafish larvae. Lines and error bars represent median ± IQR. p values were derived from Mann-Whitney tests. Data are representative of three independent experiments. See also Supplementary Fig. S7.