Figure 2

Cardiac damage increased estrogen expression in zebrafish. (A, B, C, D, E, F and G) qRT-PCR showing the expression of estrogen receptor genes in the heart (A, B) and fin (E, F) of zebrafish 7 days after CI; and plasma E2 concentration in zebrafish with heart (C, D) or fin (G) injury at 7 days. n = 3, two-tail t test (A, B, E, F, G), and one-way ANOVA with LSD post hoc test (C, D), *P < 0.05, **P < 0.01, n.s, not significant. (H) Detection of vitellogenin by Western blotting of plasma collected from three independent untreated male zebrafish and fish one day after SO and CI. (I) Detection of VTG (red) in untreated male zebrafish and in male zebrafish on day 7 after heart CI and SO. Scale bars: 1 mm. (J) Detection of VTG in uninjured caudal fin and on 7 days after fin amputation. The dashed white line showed the shape of fin, and the white line showed the amputation site. Scale bar: 1 mm. Un, untreated; SO, sham-operation; and CI, cryoinjury.