FIGURE

Figure 3

ID

ZDB-FIG-200226-12

Publication

Merrigan et al., 2020 - Calcitriol and non-calcemic vitamin D analogue, 22-oxacalcitriol, attenuate developmental and pathological choroidal vasculature angiogenesis ex vivo and in vivo

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

Vitamin D<sub>3</sub> analogue attenuates mouse choroidal sprouting angiogenesis

Mouse RPE-choroidal fragments were cultured in Matrigel^® for 24 h and further cultured with 10 μM 22-oxacalcitriol for 6 days. On day 7, samples were fixed and sprouting area quantified from phase contrast images using ImageJ freehand tool. (A) 22-oxacalcitriol and positive control sunitinib significantly attenuated choroidal sprouting angiogenesis. Graph showing mean percent sprouting area compared to vehicle control ± SEM; statistical analyses were performed using one-way ANOVA with Dunnett’s post hoc test; asterisk indicates ^*p≤0.05 and ^***p≤0.001 and n = 3 with up to 6 replicates per individual experiment. (B) Representative brightfield images of mouse choroidal sprouting after 7 days with indicated treatments. (C) Representative calcein stained images of mouse choroidal sprouting after 7 days with indicated treatments. Calcein staining of RPE-choroidal cultures confirms explant viability in vehicle and 22-oxacalcitriol treated explants. Scale bar represents 0.5 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oncotarget