FIGURE

Figure 9

ID

ZDB-FIG-200211-9

Publication

Swartz et al., 2019 - Novel ethanol-sensitive mutants identified in an F3 forward genetic screen

Other Figures

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Figure 9

Craniofacial defects in au29 mutants. In untreated au29 mutants (A, B), there are reductions to the ethmoid plate in the neurocranium (A, arrows). Within the viscerocranium, the ceratohyal cartilages (arrow) fail to project anteriorly and the number of ceratobranchial cartilages (numbered) is reduced (B). EtOH exposure dramatically exacerbates these phenotypes (C, D). The ethmoid plate is greatly reduced in EtOH‐treated au29 mutants (C, arrow; the eyes have been dissected away to more clearly visualize the cartilage). (E) Schematic representation of the au29 and au113 alleles. Schematic of exons 14 through 21, of 24 predicted exons, is shown with the location of au29 and au113 noted. The splice donor of exon 15, underlined in wt sequence, is mutated in au29. A 5‐bp indel is present in au113. The PAM sequence is underlined in the wt sequence, and the FspI site used for genotyping is shown in red.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	si:dkey-88l16.3^au29/au29 (AB)
Condition:	chemical treatment: ethanol
Observed In:	ceratobranchial cartilage ceratohyal cartilage cranial skeletal system development cranium ethmoid cartilage
Stage:	Day 5

Phenotype Detail

Acknowledgments

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