FIGURE

Fig. 6

ID

ZDB-FIG-200207-17

Publication

Mourabit et al., 2019 - New insights into organ-specific oxidative stress mechanisms using a novel biosensor zebrafish

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Fig. 6

Concentration-dependent tissue response in Tg (3EpRE:hsp70:mCherry) - zebrafish larvae exposed to Cu2+. Fish larvae were exposed to either a control (0.83??g/L Cu2+; Ai-iv) or three concentrations of Cu2+ (B; C; Di-iv) from 2 to 4?dpf and subsequently imaged via epifluorescence and confocal microscopy. Confocal microscopic images of fixed larvae under control (Aii-iv) and 6.46??g/L Cu2+ (Dii-iv) conditions; green fluorescence indicates staining with an anti-Na+ K+ ATPase antibody (alpha5), red fluorescence is EpRE-mCherry signal, and yellow indicates co-localised signal. Arrowheads?=?ionocytes. For images (A?D), fluorescence intensity is represented using a ?Fire? lookup table with a 0?255 pixel intensity calibration bar. Measured Cu2+ culture medium concentrations displayed. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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