ZFIN ID: ZDB-FIG-200206-8
Hin et al., 2020 - Accelerated brain aging towards transcriptional inversion in a zebrafish model of the K115fs mutation of human PSEN2. PLoS One   15:e0227258 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig 8 Cells expressing L-plastin are more abundant across the heterozygous mutant (<italic>psen1</italic><sup><italic>K97fs/+</italic></sup>) zebrafish brain than in wild type siblings at 24 months.

Immunostaining for the pan-leukocyte marker L-plastin supports increased numbers of microglia in the forebrain (A-B), midbrain (C-D) and hindbrain (E-F). Increased microglial abundance is evident in psen1K97fs/+ heterozygotes in both ventricular (D.i, F.i) and parenchymal (D.ii) regions compared to wild types (C, E). (G) Significant differences in MFI were observed between the forebrain, midbrain and hindbrain of psen1K97fs/+ and psen1+/+ fish; **p = 0.0048, ***p = 0.0005, ****p < 0.0001; two-way ANOVA with Sidak’s multiple comparisons test. Data presented as means with SEM. Scale bar 50 μm in all images.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-lcp1 IHC TU standard conditions Adult forebrain microglial cell
IHC Adult hindbrain microglial cell
IHC Adult midbrain microglial cell
IHC psen1uaa10/+ standard conditions Adult forebrain microglial cell
IHC Adult hindbrain microglial cell
IHC Adult midbrain microglial cell
Phenotype Details
Fish Conditions Stage Phenotype
psen1uaa10/+ standard conditions Adult forebrain microglial cell increased amount, abnormal
Adult forebrain microglial cell ab2-lcp1 labeling increased amount, abnormal
Adult hindbrain microglial cell ab2-lcp1 labeling increased amount, abnormal
Adult hindbrain microglial cell increased amount, abnormal
Adult midbrain microglial cell increased amount, abnormal
Adult midbrain microglial cell ab2-lcp1 labeling increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One