FIGURE

Fig. 3

ID

ZDB-FIG-200115-46

Publication

Park et al., 2019 - Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut

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Fig. 3

The Cubn/Amn Endocytic Receptor Complex Mediates Protein Uptake in LREs

(A) Live confocal images showing LY and mCherry uptake in LREs of cubn^pd1169 +/+, +/−, −⁄− 6 dpf larvae. Larvae were imaged 5 h after gavage. cubn ^pd1169 mutants show selective loss of protein (mCherry) internalization. Scale bar, 50 μm.

(B and C) Internalization profiles of LY (left) and mCherry (right) of 6dpf cubn ^pd1169 +/+ (n = 5), +/− (n = 10), −⁄− (n = 9) (B) and of amn ^pd1189+/+ (n = 4), +/− (n = 15), −⁄− (n = 4) (C). Endocytic receptor mutants cubnand amn exhibited specific defects in protein uptake (arrows). Data are means ± S.E.M. See also Figures S5 and S6.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	amn^{pd1189/pd1189} cubn^{pd1169/pd1169}
Observed In:	lysosome-rich enterocyte protein import
Stage:	Day 6

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 51(1), Park, J., Levic, D.S., Sumigray, K.D., Bagwell, J., Eroglu, O., Block, C.L., Eroglu, C., Barry, R., Lickwar, C.R., Rawls, J.F., Watts, S.A., Lechler, T., Bagnat, M., Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut, 7-20.e6, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell