Fig. 4

Molecular effects of phytochemicals on TNBC cells. a Representative human phospho-kinase blot array of BT-549 cells under no treatment (left), fisetin treatment (middle), and quercetin treatment (right). The kinases boxed and numbered in the blots represent major signaling protein targets of the phytochemicals. b Quantified normalized dot blot intensity of multiple kinases from fisetin and quercetin treatments of BT-549 cells. Error bars represent standard error from the mean (n = 2). Heatmaps of normalized phosphorylated levels of 43 protein kinases and 2 related proteins in nine TNBC cell lines treated with (c) 200 μM fisetin and (d) 200 μM quercetin. Phosphorylated level of each protein from a treated group was normalized by that from the corresponding vehicle control group to generate the heatmaps. Signaling molecules highlighted in boxes showed significant changes in phosphorylation by fisetin and quercetin treatments