Figure 6

Colony-forming assays of HSPC populations. (a) Representative images of a colony formed in the presence of Epoa (CFU-E) or Gcsfb (CFU-G). (b,c) Percentage (b) and absolute number (c) of CFU-E (red bars) and CFU-G (blue bars) in gata2a⁺runx1⁺, gata2a⁻runx1⁺, or gata2a⁺runx1⁻ cells in the kidney. Data are shown as mean ± s.e.m. *p < 0.01; **p < 0.001 by one-way ANOVA with Dunnett’s test. (d) Representative image of a large colony observed in the well plated with gata2a⁺runx1⁺ cells in the presence of Gcsfb. The image shows cells expressing BFP. (e,f) Representative images of a colony formed by gata2a⁺runx1⁺ cells in the presence of both Epoa and Gcsfb. Orange, red, green, and black arrowheads in (f) indicate gata2a⁺runx1⁺, gata2a⁻runx1⁺, gata2a⁺runx1⁻, and gata2a⁻runx1⁻ cells, respectively. (g) Percent distribution of individual GFP⁺ mCherry⁺ (orange bar), GFP⁻ mCherry⁺ (red bar), GFP⁺ mCherry⁻ (green bar), and GFP⁻ mCherry⁻ (black bar) cells at day 0, 3, and 7 of culture. Bars, 20 μm (a), 100 μm (d), 5 μm (e,f).