Fig. 1

McCarroll et al., 2019 - Zebrafish behavioural profiling identifies GABA and serotonin receptor ligands related to sedation and paradoxical excitation
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Fig. 1

GABAAR PAMs enhance acoustic startle in zebrafish. Zebrafish were treated with the indicated compounds and analyzed for changes in behavioral responses. a The scatter plot quantifies acoustic startle response as a z-score (y-axis) in zebrafish treated with the indicated CNS-depressants (x-axis) at the indicated concentrations (colorbar). Each point represents the average of n = 12 wells and 6 experimental replicates (also listed in Supplementary Table 1). b These plots show how the indicated compounds impact zebrafish motor activity (y-axis) over time (x-axis) (n = 12 wells, shaded boundary = 95% confidence interval; nMI, normalized motion index). Colored bars above the x-axis represent the timing and duration of low-volume acoustic stimuli (gray bars) and violet light stimuli (purple bars). The vertical dotted line indicates where the first assay ends and the second begins. c Representative images of animals treated with the indicated compounds. Time stamps indicate the time elapsed from the initial presentation of a low-volume acoustic stimulus. d These plots compare the motor activity (y-axis) over time (x-axis) of animals treated with DMSO (gray) or etomidate (red) (n = 50 larvae). Consecutive stimuli (n = 60) are indicated by vertical gray bars. e Dose-response curve showing phenoscores at the indicated concentrations (each point represents n = 12 wells/dose, error bars: ± SD). f Bar plot showing normalized response to the indicated stimulus (tap or violet light) of animals treated with DMSO, 6 μΜ propofol, or 6 μΜ etomidate (n = 12 wells, error bars: ± SD) for the indicated durations. g Average phenoscores (y-axis) of zebrafish treated with the indicated compounds. Dashed lines intersecting the y-axis at 0.51 and 0.71 correspond respectively to 1% and 5% significance cutoffs, as determined from statistical simulations. Compounds are grouped by ligand class: (1) GABABR agonist, (2) GABAAR orthosteric agonist, (3) PAM of δ-subunit containing GABAARs, (4) GABAAR BZ-site PAM, (5) GABAAR non-BZ-site PAM, (6) GABAAR neurosteroid PAM, (7) GABAAR anesthetic PAM

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
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