FIGURE

FIGURE 15

ID

ZDB-FIG-191230-51

Publication

König et al., 2019 - Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish

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FIGURE 15

Inhibition of Serotonin production by pcpa-treatment does not prevent HCS-cell regeneration. (A,B) Whole mount immunofluorescent staining for 5-HT and SV2 in pcpa-treated (B) and control (A) fins at 3 dpa. Pcpa treatment eliminates serotonin, but does not eliminate SV2 expression in HCS-cells. (A’,B’) The treatment does not prevent HCS-cell regeneration. (C,D) Whole mount immunofluorescence staining for Calretinin and SV2 in pcpa-treated (D) and control (C) fins at 3 dpa. Pcpa-treatment does not suppress the Calretinin expression in SV2-positive cells (C’,D’). (E) Density of 5-HT-positive cells in pcpa-treated fins decreased dramatically as compared to control fins, validating the activity of pcpa (72 ± 21 vs. 12 ± 10 cells per mm²). N ≥ 3. (F) Density of SV2-positive cells is similar in control and pcpa-treated fins (106 ± 26 vs. 92 ± 47 cell per mm²). N ≥ 3. (G) Density of Calretinin-positive cells remains unaltered despite of inhibition of serotonin production by the pcpa-treatment (126 ± 12 vs. 107 ± 44 cells per mm²). N ≥ 3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.