ZFIN ID: ZDB-FIG-191230-509
Xin et al., 2019 - Cell-autonomous regulation of epithelial cell quiescence by calcium channel Trpv6. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Day 4 to Day 5

Figure 3 <italic>trpv6</italic>, <italic>igf1ra</italic>, <italic>igf1rb</italic> expression in <italic>trpv6</italic><sup>-/-</sup>.

(A–C) trpv6Δ7+/-; Tg(igfbp5a:GFP) intercrosses were raised in normal or low [Ca2+] solutions from 3 to 5 dpf. Caudal fin was clipped for genotyping and 4–6 fish from the same genotype group were pooled for RNA extraction and RT-qPCR. Gene expression level was normalized to 18 s. Values shown are Mean ± SEM, n = 3.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-rps6 IHC trpv6mi604/mi604; mi602Tg control Day 4 integument
IHC Day 4 NaK ionocyte
IHC trpv6mi604/mi604; mi602Tg chemical treatment: BMS-754807 Day 4 integument
IHC Day 4 NaK ionocyte
IHC trpv6mi605/mi605; mi602Tg control Day 4 integument
IHC Day 4 NaK ionocyte
IHC trpv6mi605/mi605; mi602Tg chemical treatment: BMS-754807 Day 4 integument
IHC Day 4 NaK ionocyte
Ab5-akt IHC trpv6mi604/mi604; mi602Tg control Day 4 integument
IHC Day 4 NaK ionocyte
IHC trpv6mi604/mi604; mi602Tg chemical treatment: BMS-754807 Day 4 integument
IHC Day 4 NaK ionocyte
IHC trpv6mi605/mi605; mi602Tg control Day 4 integument
IHC Day 4 NaK ionocyte
IHC trpv6mi605/mi605; mi602Tg chemical treatment: BMS-754807 Day 4 integument
IHC Day 4 NaK ionocyte
Ab9-mapk IHC mi602Tg chemical treatment: U0126 Day 4 whole organism
IHC trpv6mi604/+; mi602Tg chemical treatment: U0126 Day 4 whole organism
IHC trpv6mi604/mi604; mi602Tg control Day 4 whole organism
IHC trpv6mi604/mi604; mi602Tg chemical treatment: U0126 Day 4 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
mi602Tg chemical treatment: U0126 Day 4 whole organism ab9-mapk labeling decreased amount, abnormal
mi602Tg low calcium Day 5 NaK ionocyte increased amount, abnormal
trpv6mi604/+; mi602Tg chemical treatment: U0126 Day 4 whole organism ab9-mapk labeling decreased amount, abnormal
trpv6mi604/mi604; mi602Tg control Day 4 integument ab5-akt labeling increased distribution, abnormal
Day 4 integument ab2-rps6 labeling increased distribution, abnormal
Day 4 NaK ionocyte ab2-rps6 labeling increased amount, abnormal
Day 4 NaK ionocyte ab5-akt labeling increased amount, abnormal
Day 4 whole organism ab9-mapk labeling increased amount, abnormal
Day 5 NaK ionocyte increased amount, abnormal
trpv6mi604/mi604; mi602Tg chemical treatment: BMS-754807 Day 4 integument ab5-akt labeling amount, ameliorated
Day 4 integument ab2-rps6 labeling amount, ameliorated
Day 4 NaK ionocyte ab2-rps6 labeling amount, ameliorated
Day 4 NaK ionocyte ab5-akt labeling amount, ameliorated
Day 5 NaK ionocyte amount, ameliorated
trpv6mi604/mi604; mi602Tg chemical treatment: U0126 Day 4 whole organism ab9-mapk labeling amount, ameliorated
trpv6mi605/+; mi602Tg low calcium Day 5 NaK ionocyte increased amount, abnormal
trpv6mi605/mi605; mi602Tg control Day 4 integument ab5-akt labeling increased distribution, abnormal
Day 4 integument ab2-rps6 labeling increased distribution, abnormal
Day 4 NaK ionocyte ab5-akt labeling increased amount, abnormal
Day 4 NaK ionocyte ab2-rps6 labeling increased amount, abnormal
Day 5 NaK ionocyte increased amount, abnormal
trpv6mi605/mi605; mi602Tg chemical treatment: BMS-754807 Day 4 integument ab2-rps6 labeling amount, ameliorated
Day 4 integument ab5-akt labeling amount, ameliorated
Day 4 NaK ionocyte ab2-rps6 labeling amount, ameliorated
Day 4 NaK ionocyte ab5-akt labeling amount, ameliorated
Day 5 NaK ionocyte amount, ameliorated
trpv6mi605/mi605; mi602Tg chemical treatment: sirolimus Day 5 NaK ionocyte amount, ameliorated
trpv6mi605/mi605; mi602Tg chemical treatment: U0126 Day 5 NaK ionocyte amount, ameliorated
trpv6mi605/mi605; mi602Tg chemical treatment: wortmannin Day 5 NaK ionocyte amount, ameliorated
trpv6mi605/mi605; mi602Tg low calcium Day 5 NaK ionocyte increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife