FIGURE

Figure 5.

ID

ZDB-FIG-191230-323

Publication

Jacobs et al., 2019 - Notch signalling maintains Hedgehog responsiveness via a Gli-dependent mechanism during spinal cord patterning in zebrafish

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Figure 5.

Inhibition of Notch signalling results in loss of Hh response followed by loss of neural progenitor identity.

(A) Wild-type embryos were incubated in LY-411575 from 20 hpf for 1, 2 or 3 hr and DMSO for the entire duration. Embryos were stained for the expression of ptc2 or sox2. Brackets in lateral views and dotted lines in transverse views denote the extent of the spinal cord. Arrows indicate ptc2 expression in the somites. The n number for each staining is shown. Scale bars: 20 μm. (B) Transverse sections of embryos were taken from the experiment in A and the extent of the expression domain of ptc2 and sox2 was measured and quantified as a percentage of the spinal cord. To directly compare changes in ptc2 and sox2 expression domains, the mean of the DMSO treated group was used as the ‘control maximum’ and all values were normalized as a percentage of their relevant control maximum. Each data point represents the average expression domain percentage of one embryo. n = 8 embryos per condition. Data are plotted with mean ± SD. Statistics: Mann-Whitney U test. Asterisks representation: p-value<0.001 (***).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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