Figure 3 supplement 1—source data 1.

Ventricular size is increased following induction of Vegfaa-OE (n_Vegfaa-VEH = 9, n_Vegfaa-OE = 10, *p<0.01) (a), but remains unchanged in cxcr4a^-/- mutants (c) (n_wt = 4, n_cxcr4a^-/-=4). (b) Lymphatic sprouts (red) follow coronary vessels, labeled by intravascular injection of Qdot705 (cyan), in 12 wpf (fish size 19–22 mm) Vegfaa-OE hearts (n = 4) (d, e) Color-coded maps of vessel thickness show mis-patterned coronary arteries and absent major OFT vessels (e, asterisk) in cxcr4a ^-/-, as compared to wt siblings (d, yellow arrow). (f) cxcr4a ^-/- hearts display partially perfused blood coronary vessels (yellow arrows) following intravascular injection of Qdots705 at 25wpf (25–30 mm) (n = 4). (g) The number of ventricular lymphatic sprouts is reduced in 22 wpf (fish size 25–28 mm) Tg(flt1_9a_cFos:GFP);Tg(lyve1b:dsRed2) cxcr4a^-/- hearts (n_wt = 4, n_cxcr4a^-/-=4, *p<0.01). (h,i) Quantification of the number of major blood vessels in 19–23 wpf (fish size 25–30 mm) wt sibling and flt4 ^-/- hearts (h) (n_wt = 5, n_flt4 = 6) or 24 wpf (fish size 25–30 mm) wt sibling and vegfc^+/- hearts (i) (n_wt = 3, n_{vegfc +/-}=5). (j,k) Quantification of ventricular size in 19–23 wpf (fish size 25–30 mm) wt sibling and flt4 ^-/- hearts (j) (n_wt = 4, n_flt4 = 5) and in 24 wpf (fish size 25–30 mm) wt sibling and vegfc ^+/- animals (k) (n_wt = 3, n_vegfc^+/-=5). (l) 12 wpf (fish size 19–22 mm) Vegfaa-VEH;vegfc^+/- fish in the background of Tg(fli1:EGFP);Tg(lyve1b:dsRed2). (m) Increased ventricular size is not reverted in 12 wpf (fish size 19–22 mm) Vegfaa-OE; vegfc^+/- animals (n_Vegfaa-VEH = 5, n_{Vegfaa-VEH;vegfc^+/-}=5, n_{VegfAa-OE-lymphatic vessel coverage}=10 n_{Vegfaa-OE;vegfc^+/-lymphatic vessel coverage} = 9, *p<0.01, relative to Vehicle treated sibling control). Error bars are 200 µm, mean ± S.E.M. All panels show anterior views.

Figure 3—figure supplement 1—source data 1.