Fig. 5

HPS1^{KD ATG}, HPS3^{KD ATG}, HPS4^{KD ATG} and HPS5^{KD ATG} induce varying degrees of glomerular damage including podocyte effacement and deposition of intracellular debris (zebra bodies). Transmission electron microscopy imaging was used to detect ultrastructural changes in glomeruli after HPS protein knockdown induced by MO injection in Tg(l-fabp:eGFP-DBP) transgene zebrafish embryos at a one to four cell stage. Collection of larvae for imaging was done at 120 hpf. (A) HPS1^{KD ATG} causes loss of glomerular endothelial fenestration and podocyte effacement. HPS3^{KD ATG} induces mild podocyte effacement. HPS4^{KD ATG} causes podocyte effacement without endothelial injury, while HPS5^{KD ATG} leads to reduced endothelial fenestration and podocyte effacement. Zebrafish injected with a control morpholino (CTRL-MO) show no sign of glomerular damage. Scale bar = 2 µm. (B) Induction of HPS protein knockdown of HPS1, -3, -4 and -5 leads to the deposition of intracellular debris primarily in podocytes. Scale bar = 500 nm.