Fig. 1

Global molecular changes that result in a drastic phenotypic alteration with expanded hypostome-like features upon systemic activation of Wnt signalling. Wnt-regulated genes were identified by differential gene expression (DE) profiling after inhibiting GSK-3β with Alsterpaullone (5 μM). a Cartoon depicting the experimental design for activation of Wnt signalling and collection of samples at different time points for transcriptome and phenotype analysis. b Morphological changes after 24 h and ectopic tentacle formation after 72 h of Alsterpaullone treatment (5 µM). Scale bar represents 1 mm. c Expression pattern of HvWnt3a in DMSO control and ALP-treated polyps 24 h post treatment. Scale bar represents 200 µm. d Smear plot showing logarithmic fold change of transcript expression compared to control on y-axis and log of read counts per million on x-axis. Red and Green colour dots denote significantly up- and downregulated genes with log₂ fold change cut-off of ±0.58 with a p value of 0.01 respectively; two biological replicates for each condition were used for DE analysis. e Bar plot showing the percentage of genes differentially regulated in selected GO term classes, upon activation of Wnt signalling. Numbers in the bars represent genes that exhibit altered expression over total genes of respective GO categories.