FIGURE

Fig. S4

ID
ZDB-FIG-191104-19
Publication
Liu et al., 2019 - Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking
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Fig. S4

(A) Time-lapse confocal images showing DFC migration in wild-type and  cxcr4a um20 mutant embryos on a  Tg(sox17: GFP) background from 75%?90% epiboly stages. Scale bar, 50 ?m. (B) Sox17 expression was examined by in situ hybridization in wild-type and  cxcr4a um20  mutants at the 75% epiboly stage. (C?D) Wild-type and  cxcr4a-deficient  Tg(sox17: GFP) embryos were harvested at the 10-somite stage for immunostaining. KV cells were labeled using an antibody against GFP. Expression of the basal?lateral marker E-cadherin (C) and the apical marker aPKC (D) were visualized using the indicated antibodies. Scale bar, 20 ?m. aPKC, atypical protein kinase; DFC, dorsal forerunner cell; GFP, green fluorescent protein; KV, Kupffer?s vesicle;  sox, SRY-box transcription factor; Tg, transgene.
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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