FIGURE

Fig. 1

ID

ZDB-FIG-191024-2

Publication

Djannatian et al., 2019 - Two adhesive systems cooperatively regulate axon ensheathment and myelin growth in the CNS

Other Figures

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Fig. 1

Loss of adhesion molecules of the paranodal axo-glial junction results in neuronal cell body wrapping. a CNS myelin of 3, 4 and 10 dpf wild-type (wt) and caspr^?/? fish. Note cell body wrappings in caspr^?/? fish. b Cell body wrappings per myelinated area at 3, 4, and 10/11 dpf ( n?=?6?10, Kruskal Wallis ANOVA: 3 dpf: p?=?0.0004, 4 dpf and 10/11 dpf: p?<?0.0001). c Cell body wrappings in wt fish ( n?=?8?9). Data derived from panel ( b). Mann-Whitney test, * p?=?0.0399. d HuC:mCherry expression in 4 dpf caspr^?/? mag^?/? fish shows neuronal cell bodies enwrapped by myelin membrane. e SEM images of cell body wrappings in cntn1b^?/? fish at dpf 10. f Sheath retractions (arrow) from neuronal cell bodies (30?min intervals, 15?16?h time lapse experiments, n?=?3, Kruskal-Wallis one-way ANOVA: p?=?0.4393). Images ( a, d, f) are maximum intensity projections of Tg(mbp:EGFP-CAAX) zebrafish dorsal spinal cord. p values, *?<?0.05, **?<?0.01, ***?<?0.001. Data are presented as means?�?s.d.Scale bars, 5?mm ( a, d, f), 2?�m ( e). Source data are provided as a Source Data file

Expression Data

Gene:	EGFP
Fish:	ue2Tg cntnap1^{tum205/tum205}; ue2Tg
Anatomical Term:	spinal cord myelin sheath
Stage Range:	Protruding-mouth to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cntn1b^{tum204/tum204}; ue2Tg cntnap1^{tum205/tum205}; ue2Tg mag^{tum202/tum202}; ue2Tg nfascb^{tum203/tum203}; ue2Tg
Observed In:	spinal cord central nervous system myelin formation spinal cord myelin sheath spinal cord neuronal cell body
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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