FIGURE

Fig. S1

ID

ZDB-FIG-191018-10

Publication

Rocha-Martins et al., 2019 - De novo genesis of retinal ganglion cells by targeted expression of Klf4 in vivo

Other Figures

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Fig. S1

Characterization of conditional Klf4 mice and zebrafish knockout. (A) Klf4 RNA content from E15.5 to P60 in wild type mice. (B) qRT-PCR for Klf4 of RNA isolated from Klf4CTR and Klf4Cre. (C) Measurements of eye volume in Klf4CTR (n=8) and Klf4Cre (n=5). (D, E) Eyes from Klf4CTR and Klf4Cre show no apparent difference. (F, G) Histological comparison between retinal sections from Klf4CTR and Klf4Cre. (H, I) No difference is seen in the optic nerve or optic chiasma. (J, K) Zebrafish embryos 48hpf from Klf4+/+ and Klf4del/del genotypes. (L) Detailed characterization of deletion and comparison with aligned sequences from mice and humans. Scale bar = 500 μm (D, E, H, I), 25 μm (F, G) or 200 μm (J, K).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	klf4^cbg14/cbg14 (AB)
Observed In:	whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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