Fig. 5-S1

(A–D) Representative images of PCNA (red) immunofluorescence showing 2 days’ treatment of RO4929097, a putative Notch inhibitor, results in more RG (PCNA⁺, white arrowheads) proliferation in injured (B) and uninjured (D) optic tecta. (A and C) The representative images of injured and uninjured optic tecta treated with DMSO. (E–E₃) Representative images of Myc (red) and PCNA (green) immunofluorescences showing a single RG with NICD-Myc overexpression (red cell, white arrowheads) is lack of PCNA expression (green signals) while many neighboring cells (green cells, open white arrowheads) without NICD-Myc expression are proliferative. This is the most common NICD-Myc and PCNA expression pattern in the heat shock-induced Notch over-activation experiments. (F–F₄) Representative images of her4.1:dRFP (red), PCNA (green) and DAPI (gray) immunofluorescences showing that the boundary (white dashed lines and yellow arrowheads) of PGZ and TS could be unambiguously defined by the DAPI signal. The cells under the boundary are her4.1:dRFP⁺, indicating their glial identity. The lack of PCNA expression in them indicates they are largely dormant. (G–G₃) Representative images of gfap:GFP (green) and DAPI (magenta) immunofluorescences showing that the boundary (white dashed lines and yellow arrowheads) of PGZ and TS could be unambiguously defined by the DAPI signal. White dashed lines represent the tectal ventricle boundary. RG, radial glia; TeO, tectal opticum; PGZ, periventricular gray zone; TS, torus semicircularis, Val, valvula cerebelli. Scale bars, 100 μm (A–D, F and G); and 20 μm (E–E₃, F₁–F₄ and G₁–G₃).