FIGURE

Fig. 6

ID

ZDB-FIG-190923-2

Publication

Wang et al., 2019 - A long noncoding RNA cluster-based genomic locus maintains proper development and visual function

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Fig. 6

CNIB1 mediates visual function via lmx1bb in zebrafish. (A) In situ hybridization of lmx1bb (indicated by arrowheads) in zebrafish offspring of CNIB1^+/+ and CNIB1^+/− 2 incross at 48 hpf. Scale bars: 400 μm. (B) Relative expression level of lmx1bb mRNA detected by RT-qPCR in the indicated zebrafish offspring (CNIB1^+/+ or CNIB1^+/− 2) that were either Control-injected or injected with in vitro transcribed CNIB1 sense (CNIB1_S) or antisense RNAs (CNIB1_AS). The data were normalized to β-actin. (C) Representative images of Western blot for Lmx1bb and β-actin in CNIB1^+/+ zebrafish with the indicated modifications. Control MO, mismatch lmx1bb MO; lmx1bb MO, lmx1bb morphants; lmx1bb MO+lmx1bb, co-injection of lmx1bbmorphants and in vitro transcribed lmx1bb mRNA. (D) Quantified expression level of Lmx1bb protein (normalized to β-actin) in the zebrafish shown in C, n is 30 in each group. (E) Gain value of the OKR assay in the zebrafish shown in C. Control-injected, n = 6; Control MO, n = 6; lmx1bb MO, n = 6; lmx1bb MO + lmx1bb, n = 6. (F) Representative images of Western blot for Lmx1bb and β-actin in zebrafish offspring from either CNIB1^+/+ or CNIB1^+/− 2 incross with the indicated modifications. lmx1bb, injection with in vitro transcribed lmx1bbmRNA. (G) Quantified expression levels of Lmx1bb protein (normalized to β-actin) in the zebrafish shown in F, n = 20. (H) Gain value of the OKR assay in the zebrafish shown in F. Control-injected CNIB1^+/+, n = 12; lmx1bb CNIB1^+/+, n = 12; Control-injected CNIB1^+/− 2, n = 9; lmx1bb CNIB1^+/− 2, n = 10.

Expression Data

Gene:	lmx1bb
Fish:	AB lnc.cnib1^zf3099/+
Anatomical Terms:	brain eye
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-lmx1bb lnc.cnib1^zf3099/+
Knockdown Reagent:	MO1-lmx1bb
Observed In:	brain eye optokinetic behavior
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.